Asked by: Alesandro Reinink
asked in category: General Last Updated: 7th February, 2020

What is cDNA used for?

In genetics, complementary DNA (cDNA) is DNA synthesized from a single-stranded RNA (e.g., messenger RNA (mRNA) or microRNA (miRNA)) template in a reaction catalyzed by the enzyme reverse transcriptase. cDNA is often used to clone eukaryotic genes in prokaryotes.

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Herein, why do we need cDNA?

cDNA is the result of reverse transcription by enzymes called reverse transcriptases. This has great significance mostly in the selective amplification of eukaryotic DNA. Now, being an exact copy of the genomic DNA, this cDNA can serve the purpose of the template DNA for in vitro amplification and subsequent analyses.

Secondly, what is the difference between DNA and cDNA? DNA is a generic term for deoxyribonucleic acid, which makes up genetic material. cDNA is synthetic DNA, ”complementary DNA, ” made using RNA reverse transcriptase on an mRNA sample template. This cDNA can then be cloned in a phage or plasmid to create a library of the expressed RNAs in the sample.

Herein, why we use cDNA instead of DNA?

When scientists use viral enzymes to make cDNA from RNA isolated from the cells and tissues that they are studying, it does not contain introns due to being spliced out in mRNA. cDNA also does not contain any other gDNA that does not directly code for a protein (referred to as non coding DNA).

Why is complementary DNA used in recombinant DNA technology?

A genomic library is created by isolating DNA from cells and then amplifying it using DNA cloning technology. To create a cDNA library, these mRNA molecules are treated with the enzyme reverse transcriptase, which is used to make a DNA copy of an mRNA. The resulting DNA molecules are called complementary DNA (cDNA).

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