Asked by: Salamu Castrillon
asked in category: General Last Updated: 23rd June, 2020

What is a CPT tube?

The BD Vacutainer® CPT™ Cell Preparation Tube with Sodium HeparinN is an evacuated Tube intended for the collection of whole blood and the separation of mononuclear cells. This configuration permits cell separation during a single centrifugation step.

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Regarding this, what is EDTA in blood collection tubes?

EDTA stands for Ethylenediaminetetraacetic acid. EDTA functions by binding calcium in the blood and keeping the blood from clotting. BD Vacutainer Plus Blood Collection Tubes contain K2EDTA, which is spray-dried to the walls of the tube.

Similarly, what is Ficoll Paque? Ficoll is a neutral, highly branched, high-mass, hydrophilic polysaccharide which dissolves readily in aqueous solutions. Ficoll radii range from 2-7 nm. Ficoll is part of Ficoll-Paque, which is used in biology laboratories to separate blood to its components (erythrocytes, leukocytes etc.).

Also, what is Vacutainer tubes?

A Vacutainer blood collection tube is a sterile glass or plastic test tube with a colored rubber stopper creating a vacuum seal inside of the tube, facilitating the drawing of a predetermined volume of liquid.

How do I isolate PBMC?

  1. Gently homogenize the blood sample inside heparin blood collection tube.
  2. Add the whole blood to conical tube that contain 4 ml of PBS (equal volume to the sample; 1:1)
  3. Homogenize or mix the solution.
  4. Add the entire solution to a new conical tube containing 4ml ficoll solution.

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