Asked by: Melito Baranda
asked in category: General Last Updated: 1st May, 2020

What is a biochemical test in microbiology?

Biochemical tests are the tests used for the identification of bacteria species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one type of organism to another.

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Likewise, what is the purpose of biochemical tests?

The purpose of biochemical tests is to identify microorganisms. This is because different microorganisms have specific metabolic profiles.

Secondly, how do you perform a biochemical test? Test Procedure

  1. Dilute your organism in a tube of sterile water to obtain a turbidity equivalent to the 0.5 McFarland test standard.
  2. Using a sterile 1mL pipette, place 1 mL of organism into the middle of the tube.
  3. Cap tightly; do not jostle.
  4. Incubate for 24 hours at 37°C.

Also know, what are the biochemical test for bacteria identification?

2.2. Biochemical tests are among the most important methods for microbial identification. Routine biochemical tests include tests for carbohydrate fermentation (Figure 2.18(A)), methyl red (Figure 2.18(B)), citric acid utilization (Figure 2.18(C)), and hydrogen sulfide production (Figure 2.18(D)).

Is a Gram stain a biochemical test?

Although described more than a century ago, the Gram stain remains the most frequently used rapid diagnostic test, and in conjunction with various biochemical tests is the cornerstone of the clinical laboratory. First described by Danish pathologist Christian Gram in 1884 and later slightly modified.

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