Asked by: Aboubakr Rodhamasked in category: General Last Updated: 26th February, 2020
What does Elisa stand for quizlet?
Similarly, you may ask, what does Elisa stand for?
Enzyme-linked immunosorbent assay (ELISA): ELISA stands for "enzyme-linked immunosorbent assay." This is a rapid immunochemical test that involves an enzyme (a protein that catalyzes a biochemical reaction). It also involves an antibody or antigen (immunologic molecules).
Additionally, why are enzymes used in this immunoassay quizlet? Enzymes provide a way to see whether the primary antibody has attached to its target (antigen) in the microplate well. Primary and secondary antibodies are invisible, so a detection method is necessary. The enzyme HRP is linked to the second antibody.
Thereof, when performing a serum Elisa procedure the sample wells are first coated with?
Plastic microtiter wells are coated with protein antigens derived from the pathogen. The microtiter wells are blocked with an irrelevant protein (such as bovine serum albumin) to prevent non-specific binding of patients' antibodies. The patients' serum samples are added to the microtiter wells and incubated.
Why would the Elisa technique be valuable for identifying viruses quizlet?
It is simple, highly sensitive, and its ability for testing large numbers of samples.