Asked by: Xuefen Hantzschmann
asked in category: General Last Updated: 9th January, 2020

How do you calculate a pipette error?

Calculate the accuracy of the pipette.
To calculate the accuracy you can use the equation A = 100 x Vavg/V0, where A is the accuracy of the pipette, Vavg is the average calculated volume and V0 is the value you set the pipette to dispense. Accuracy should be between 99-101%.

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In this manner, how do you find the percent error of a pipette?

Calculate the percent error of your measurement.

  1. Subtract one value from the other: 2.68 - 2.70 = -0.02.
  2. Depending on what you need, you may discard any negative sign (take the absolute value): 0.02.
  3. Divide the error by the true value:0.02/2.70 = 0.0074074.
  4. Multiply this value by 100% to obtain the percent error:

Similarly, how do you calculate uncertainty? To begin, simply square the value of each uncertainty source. Next, add them all together to calculate the sum (i.e. the sum of squares). Then, calculate the square-root of the summed value (i.e. the root sum of squares). The result will be your Combined Uncertainty.

Similarly, it is asked, what is the uncertainty of a pipette?

Uncertainty due to repeatability of pipetting u (V, REP) is equal to this standard deviation 0.0057 ml. Pipetting is often used in titration analysis.

How do you stop pipetting errors?

10 Ways to Prevent Pipetting Errors

  1. Practice a smooth and slow motion.
  2. Pre-wet the pipette tip.
  3. Hold the pipette vertically when drawing in liquid and at a 45 degree angle when dispensing.
  4. Immerse the tip slightly into the liquid during aspiration.
  5. Touch off the pipette on the sidewall of the container.

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